It was subsequently reformed into an administrative subdivision, also called a regiment, of the early Cossack Hetmanate on 8 September, 1649 following the Treaty of Zboriv, replacing the earlier starostwos of Pereiaslav, Cherkasy and Kaniv, as well as a part of the Kiev Voivodeship.

[1] During the Ruin, when skirmishes and conflict between different Cossack factions was common, the regiment often changed hands between different hetmans.

Eventually, after the Treaty of Bakhchisarai in 1681, the regiment was completely disbanded, after which its members settled in neighbouring regions, mostly in Left-bank Ukraine.

In 1702, a new regiment now centred around Kaniv was formed and engaged in Paliy uprising,[1] but it was once again disbanded in 1712, as part of the conditions of the Treaty of the Pruth.

According to the Zboriv register of 1649, the regimental scribe (Ukrainian: Писар) was Herasym Savych, while the Yesaul was Bohdan Shabelnychenko.