Kholosa Mthikazi Biyana (born 6 September 1994) is a South African soccer player who plays as a midfielder for SAFA Women's League club Mamelodi Sundowns Ladies and the South Africa women's national team.

[2] In 2023, she was her first club continental title with Sundowns Ladies at the 2023 CAF Women's Champions League.

[5] Biyana captained UKZN Ladies in their maiden Women's Varsity Football season in 2018.

[3][10][11][12] Biyana started for all three matches played by South Africa at the 2019 FIFA Women's World Cup.

She was on the team that made it to the Round of 16 at the 2023 FIFA Women's World Cup and started for 2 of South Africa's 4 matches.