[1][2][4][7] Gicklhorn treated this species as a colorless sulfur bacteria and called it Pseudomonas bipunctata.

Years later, Dubinina, Grabovich, and La Rivière isolated this species from the precipitates of sewage aeration tanks called the white mat.

[8] Most of the culturing procedures model Dubinina and Grabovich's 1984 article on M. bipunctata: it includes sodium acetate (1 g/L), calcium chloride (0.1 g/L), casein hydrolysate (0.1g/L), yeast extract (0.1g/L), and agar (1g/L) along with a vitamin supplement, trace elements, and FeS as a sulfide source.

[9] Macromonas bipunctata has been cultured in many studies that show H2O2 is formed in different biochemical reactions: not only in the process of respiration with the participation of enzymes of the electron transport chain, but also in the course of utilization of intracellular oxalate inclusions in the cytoplasm.

[2][3][9][10] Furthermore, in the end process of becoming a toxic metabolite, it would decompose upon chemical interaction with the reduced sulfur compounds, whose presence is characteristic for the habitat of these bacteria.

[2][3][9][10] When grown on the media containing organic acids of the TCA cycle, the unicellular sulfur bacterium M. bipunctata is able to synthesize and store calcium oxalates inside the cell.

[2][3][7] Furthermore, M. bipunctata was found that reduced sulfur compounds such as H2S were not used by the strains as electron donors, rather, their oxidation was due to interaction with H2O2.

[1][2] It is assumed that Macromonas bipunctata, at least in part, is responsible for the metabolism of organic acids and calcium deposition in the form of a calcite crystals.

Other than its communal living in moonmilk formations in certain caves, it was first isolated from a white mat formed in a waste-water.

[1][5] This microorganism is also found as a free-living microbe adapted to high-calcium and high alkaline, freshwater environments.

[8][11] Macromonas bipunctata has an indirect connection to the discovery of several antibiotics within the moonmilk formations, but its greatest importance is in its chemical cycling of minerals such as sulfur and calcium in mesophilic environments.