Magdalena of Nassau-Dillenburg (15 December 1547 at Dillenburg Castle in Dillenburg – 16 May 1633 in Öhringen) was a daughter of William I, Count of Nassau-Siegen and his second wife, Juliana of Stolberg.

Despite frequent pregnancies, she also worked in the castle's pharmacy, where she gave medicine to the poor, a practice her mother had begun.

She was described as very generous, giving money, food and medicine to the poor and the needy.

On 27 January 1567 at Dillenburg Castle, she married Count Wolfgang of Hohenlohe-Weikersheim at the age of 19.

He was a son of Louis Casimir of Hohenlohe-Waldenburg and Anna of Solms-Lich.