It runs from PTH 14 at Winkler to the U.S. border, where it becomes North Dakota State Highway 32.

Between its northern end and its intersection with PR 243, PTH 32 follows the Boundary Commission Trail.

The highway crosses a creek and heads north, joining the Boundary Commission Trail at an intersection with PR 243 and traveling through Friedensfeld West, Hochfeld (where it has a short concurrency (overlap) with PR 201), and Schanzfeld.

PTH 32 enters the city of Winkler, following 1st Street through neighborhoods and a major business district on the east side of downtown, crossing some railroad tracks and coming to an end shortly thereafter at a junction with PTH 14, with the road continuing north as PR 428 towards Roland.

Provincial Road 521 (PR 521) is an east-west spur of PTH 32 in the Rural Municipalities of Stanley and Rhineland in the Pembina Valley Region of the province, located only 1.7 kilometres (1.1 mi) north of the United States border.