The route starts at the Mississippi–Louisiana state line, and it travels northeastward from that point.

The road was designated in 1955, as part of projects initiated by the Mississippi State Highway Department (MSHD).

[1] MS 569 starts at the Mississippi–Louisiana state line, where it meets the northern terminus of Louisiana Highway 67 (LA 67).

[2] It then intersects Lower Centreville Road, which leads to the unincorporated area of Olio.

MS 569 and Lower Centreville Road are briefly concurrent to cross the Mart Branch, and the latter continues eastward.

It continues over Shaw and Causey Creeks before meeting MS 48 at a three-way junction at Beechwood.

Between the routes' intersection with Enterprise Road, it crosses over Wagoner Creek and West Fork of the Amite River.

After crossing a branch of East Fork of the Amite River, the route intersects MS 570.

[11][12] MS 569 was extended northward along a paved road to Lucien in Franklin County by 1957.