MS 613 begins in Jackson County at an intersection with US 90 in the city of Pascaguola, only about 2.0 miles (3.2 km) north of the Gulf of Mexico coastline.

It heads northeast as a four-lane undivided highway along Telephone Road through a mix of neighborhoods and business districts, where it passes by the Jackson County Fairgrounds and Civic Center at a large intersection with Shortcut Road, where it curves northward to pass through another business district to enter the neighboring city of Moss Point at an intersection with Jefferson Avenue.

The highway has an interchange with I-10 (Exit 68) before leaving Moss Point, narrowing to two-lanes, and traveling north through the community of Escatawpa and having an intersection with MS 63 directly beside Plant Daniel.

MS 613 winds its way northwest through rural farmland for several miles, passing through the communities of Agricola and Evanston, to enter the city limits of Lucedale and travel through some neighborhoods along Carter Street before turning north (right) onto Mill Street in a small business district.

The highway passes through more neighborhoods for several blocks before entering downtown and coming to an end at an intersection with MS 198 (Main Street) near the center of town.