Multienzyme complexes carry out a single or multi-step biochemical reaction taking place within cells.
A multienzyme complex that functions in the histidine biosynthesis pathway has been studied at the biochemical and genetic level in the fungus Neurospora crassa.
A gene (His-3) was found to encode a protein that functions as a multienzyme complex having three distinct enzymatic activities in the biosynthesis pathway.
This finding suggested that each of the activities of the multienzyme complex are encoded separately from each other, but within the same gene.
[2] Some His-3 mutants were also found that lacked all three activities simultaneously, suggesting that some mutations can cause loss of function of the whole multienzyme complex.