[2] It may also be prepared by digesting alloxan with alcoholic ammonia.

Justus von Liebig and Friedrich Wöhler in Giessen, Germany, had investigated the purple product, murexide, obtained from snake excrement in the 1830s, but this was not an abundant raw material, and a method of using it as a dyestuff was not established at that time.

[3] In the 1850s, French colourists and dye-producers, such as Depoully in Paris, succeeded in making murexide from abundant South American guano and of applying it to natural fibres.

It was then widely adopted in Britain, France and Germany.

Murexide is used in analytical chemistry as a complexometric indicator for complexometric titrations, most often of calcium ions,[4] but also for copper, nickel, cobalt, thorium and rare-earth metals.