Nicotryine is lesser known and minor tobacco alkaloid.

[2][4][5] It also inhibits CYP2A13 (Ki = 5.6 ± 0.86) which might play role in nicotine metabolism.

[5][6] Nicotyrine is formed by gradual oxidation of nicotine in e-liquids and causes delayed nicotine clearance and attenuated withdrawal symptoms.

[7] alpha-nicotyrine and beta-nicotyrine are positional isomers of each other.

Nicotyrine can be produced readily from nicotine by catalytic dehydrogenation[8] and from tobacco biomass by catalytic pyrolysis.