It can carry large amounts (about 100–300 kilobases) of other sequences for a variety of bioengineering purposes in bacteria.
[4] PAC has 2 loxP sites, which can be used by phage recombinases to form the product from its cre-gene recognition during Cre-Lox recombination.
This process circularizes the DNA strand, forming a plasmid, which can then be inserted into bacteria such as Escherichia coli.
[5] Electroporation allows for lysogeny of PACs so that they can replicate within cells without disturbing other chromosomes.
Some advantages of PACs compared to YACs includes easier manipulation of bacteria system, easier separation from DNA hosts, higher transformation rate, more stable inserts, and they are non-chimeric which means they do not rearrange and ligate to form new DNA strand, allowing for a user friendly vector choice.