Reflectometric interference spectroscopy

The partial beams of the white light are reflected at each phase boundary and then refracted (transmitted).

These reflected partial beams superimpose which results in an interference spectrum that is detected using a diode array spectrometer.

Chemosensors are particularly suitable for measurements under difficult conditions and in the gaseous phase.

When using biosensors, polymers such as polyethylene glycols or dextrans are applied onto the layer system, and on these recognition elements for biomolecules are immobilized.

RIfS, like SPR is a label-free technique, which allows the time-resolved observation of interaction among the binding partners without the use of fluorescence or radioactive labels.