Single colour reflectometry

The obtained binding curves using SCORE provide detailed information on kinetics and thermodynamics of the observed interaction(s) as well as on concentrations of the used analytes.

These data can be relevant for pharmaceutical screening and drug design, biosensors and other biomedical applications, diagnostics, and cell-based assays.

Binding of specific ligands to the immobilised receptor molecules results in a change refractive index n and physical thickness d of the sensitive layer.

The SCORE technology was commercialised by Biametrics GmbH, a service provider and instrument manufacturer with headquarters in Tübingen, Germany.

In January 2020, Biametrics GmbH and its technology was acquired by BioCopy Holding AG, headquartered in Aadorf, Switzerland.

Reflectometry can be either realised by 1: monitoring the shift of an extremum in x-direction via a spectrometer ( BLI , RIfS ), or 2: monitoring the change in intensity of one wavelength in y-direction via a photodiode or camera (SCORE)
1: Monochromatic light is reflected at a biolayer interface, 2: upon binding the reflection conditions change, resulting in 3: a higher reflected intensity due to positive interference
Example binding curve in an intensity vs time diagram
Multiplexed binding curves using an array format