The women's singles competition of the squash events at the 2023 Pan American Games was held from October 31 to November 1 at the Racquet Sports Center (Centro de Entrenamiento del Tenis y Deportes de Raqueta) in Santiago, Chile.

[1][2] Each National Olympic Committee could enter a maximum of three athletes into the competition.

The athletes were drawn into an elimination stage draw.

Once an athlete lost a match, they will be no longer able to compete.

Unlike other events, there is not bronze medal match, so the two athletes that do not win during the semifinals receive a bronze medal.