Canada competed at the 2023 Pan American Games in Santiago, Chile from October 20 to November 5, 2023.

In February 2023, Guadalajara 2011 and London 2012 weightlifting gold medallist Christine Girard was named as the country's Chef De Mission.

[1] On October 12, 2023 a final team of 473 athletes (208 men and 265 women) competing in 36 sports was confirmed by the Canadian Olympic Committee.

[2] Beach volleyball athletes Melissa Humana-Paredes and Brandie Wilkerson were the country's flagbearers during the opening ceremony.

[4] Meanwhile, gold medalists at the games breakdancer Philip Kim (Phil Wizard) and sprint canoeist Katie Vincent were the country's flagbearers during the closing ceremony.

[6] The Canadian team did not compete in the sports of baseball, basketball, basque pelota, football (soccer) and volleyball.

[9] Tatiana Aholou, Adam Keenan, Myles Misener-Daley and Grace Fetherstonhaugh were later withdrawn, while Kiana Gibson as added, making the final teams size 24 athletes (11 men and 13 women).

Misener-Daley declined his invitation after confusion over selection and wanting to be ready for the 2024 Summer Olympics.

Canada qualified two athletes through the 2021 Junior Pan American Games in Cali, Colombia (Brian Yang and Rachel Chan).

[13] A further eight quotas were earned by Canadian athletes, by finishing first overall in the Pan American Games ranking.

[23][24] After reallocations, Canada earned two additional quotas (one man and one woman) for a total of five spots (two men and three women).

[30] Canada qualified four BMX racers (two men and two women) through the UCI World Rankings.

[2] Canada qualified a full team of four riders in dressage, eventing and show-jumping based on FEI world rankings released on September 11, 2023.

;Women Key: Canada qualified a men's team (of 16 athletes) by finishing 3rd at the 2022 Pan American Cup.

[40] Canada qualified a women's team (of 16 athletes) by finishing 3rd at the 2022 Pan American Cup.

[45] Canada qualified a team of ten gymnasts in artistic (five men and five women) at the 2023 Pan American Championships.

[45] Canada qualified four gymnasts in trampoline (two men and two women) at the 2023 Pan American Championships.

[54] The six qualified athletes marked the most karatekas Canada has sent to an edition of the Pan American Games.

[73] Canada qualified a total of 16 quota spots (shooters) at the 2022 Americas Shooting Championships.

[78] Canada finished the games with one bronze medal by Alannah Yip in the women's boulder & lead event.

[79] Boulder & lead Speed Canada qualified a full team of six athletes (three men and three women) through the 2023 Pan American Squash Championships.

[82] However, surfer Erin Brooks could not compete for Canada due to not receive Canadian citizenship in time.

[86] A total of 11 former Olympians, including Olympic Gold medalist Maggie MacNeil were named to the team.

[89] Benjamin Cote will compete in both disciplines, meaning the team size for swimming is 38 athletes (19 per gender).

Canada qualified a full team of six athletes (three men and three women) through the 2023 Pan American Games selection tournament held in Toronto, Ontario.

[91][92] Canada qualified 9 athletes (five men and four women) during the Pan American Games Qualification Tournament.

Canada qualified a team of four triathletes (two men and two women) after finishing third at the 2023 Pan American Mixed Relays Championship.

[96] Canada also qualified one additional male and female quota, for a final team size of six athletes (three per gender).

[2] Justina Di Stasio and Alex Moore were also replaced by Shauna Kuebeck and Hunter Lee respectively due to injury.

[108] Canada qualified a men's team (of 12 athletes) by finishing fourth in the 2022 FIBA Americup.