[1] Sport shooter Vincent Hancock and artistic gymnast Jordan Chiles were the country's flagbearers during the opening ceremony.

The United States qualified one archer after winning the respective event at the 2021 Junior Pan American Games.

[3][4] Men Women Mixed The United States automatically qualified a full team of nine artistic swimmers.

Quarterfinal Semifinal Gold medal game The United States qualified a women's team (of 4 athletes) by finishing third in the 2022 FIBA 3x3 AmeriCup.

[20] Men Women The United States qualified two male and two female breakdancers at the 2023 Pan American Championships.

[24][25] The United States qualified four cyclists (two men and two women) in BMX race through the UCI World Rankings.

[28] Men Women The United States qualified a full team of four riders in dressage, eventing and show-jumping based on FEI world rankings released on September 11, 2023.

Semifinal Bronze medal game The United States qualified a women's team (of 16 athletes) by finishing fourth at the 2022 Pan American Cup.

Summary Key: The United States qualified a men's team of 18 athletes by winning the 2022 CONCACAF U-20 Championship.

Semifinal Bronze medal game The United States qualified a women's team of 18 athletes by winning the 2022 CONCACAF W Championship.

[43] The United States qualified a team of 12 gymnasts in artistic (six men and six women) at the 2023 Pan American Games.

[54][55] Men Women Mixed The United States qualified a team of two athletes in artistic skating (one man and one woman).

[62][63] Men Women Mixed The United States qualified a total of 22 shooters after the 2022 Americas Shooting Championships.

Gold medal game The United States qualified a full team of 12 climbers (six men and six women) by virtue of their IFSC world rankings.

[84] Men Women Mixed The United States qualified three athletes at Kyorugi events, by virtue of their titles in the 2021 Junior Pan American Games.

[85] The United States also qualified 10 athletes (five men and five women) during the Pan American Games Qualification Tournament.

[87] The United States qualified a team of four triathletes (two men and two women) after winning the 2023 Pan American Mixed Relays Championship.

Quarterfinal Semifinal Gold medal game The United States automatically qualified a women's team (of 11 athletes).

Quarterfinal Semifinal Gold medal game The United States qualified two wakeboarders (one man and one woman) during the 2022 Pan American Championship.