St. Anne Chapel at Old River is a historic Catholic chapel founded in the 1800s along the banks of Old River near Cypress and Isle Brevelle in Natchitoches Parish, Louisiana, serving the Old River community.

It is the cultural and religious center of the area's Louisiana Creole people, predominantly of French descent.

[3] The chapel was founded by French Creole families in the 1800s as a mission of St. Augustine Catholic Parish Church of Isle Brevelle.

During this period, St. Augustine Catholic Parish Church founded 3 other missions: St. Anne Church (Spanish Lake) (serving the Adai Caddo Indians of Louisiana), St. Joseph's Catholic Mission at Bayou Derbonne, and St. Charles Chapel at Bermuda.

[4] Notable Creole founding and patron families include Rachal, Longlois, Lacaze, Metoyer and Brevelle.