Trout Run is a tributary of East Branch Fishing Creek in Sullivan County, Pennsylvania, in the United States.

The stream is acidic and is considered by the Pennsylvania Department of Environmental Protection to be impaired by atmospheric deposition and metals.

After a short distance, it reaches its confluence with East Branch Fishing Creek.

[1] Trout Run joins East Branch Fishing Creek 3.60 miles (5.79 km) upstream of its mouth.

It could potentially improve the water quality of East Branch Fishing Creek, but not significantly.

Proposed methods of increasing the alkalinity of Trout Run include road liming.

[3] A total of 1.8 miles (2.9 km) of streams in the watershed of Trout Run are considered by the Pennsylvania Department of Environmental Protection to be impaired to do atmospheric deposition and metals.

[3] The Pennsylvania Department of Environmental Protection has designated Trout Run to be used for aquatic life.