All of US 27 in Georgia runs concurrently with State Route 1 (SR 1) and is also designated as the Martha Berry Highway.

It connects Bainbridge, Colquitt, Blakely, Cuthbert, Lumpkin, Cusseta, Columbus, LaGrange, Carrollton, Bremen, Cedartown, Rome, Summerville, LaFayette, Fort Oglethorpe, and Rossville.

US 27 heads northwest through rural southwestern Georgia, crossing into Decatur County as a four-lane divided highway, and passes Attapulgus to the east on its way to Bainbridge.

Entering Floyd County, US 27 briefly becomes a controlled-access highway with exits serving Darlington Drive/Old Lindale Road, Dean Avenue (SR 101), and East 12th Street.

Continuing through Rome as Turner McCall and Martha Berry boulevards, the highway then angles west toward Chattooga County and Summerville.

Turning sharply north again in Summerville, the highway travels through LaFayette in Walker County.

At this point, US 27 becomes LaFayette Road and then passes back and forth twice between Walker and Catoosa counties, before meeting its northern terminus at the Tennessee state line in Rossville.

SR 53 was proposed just east of LaFayette, and an unnumbered road was built north-northwest to Fort Oglegthorpe and then north to Tennessee.

[4][9] By the end of 1930, US 41W was designated on SR 1 from Rome to LaFayette and possibly on the LaFayette–Tennessee segment of the unnumbered road.

[21][22] Between July 1957 and June 1960, SR 1 was proposed to be rerouted on an eastern bypass of Summerville, from a point east-southeast of the city to Trion.

[22][30] Between June 1963 and the end of 1965, SR 1 was proposed to be rerouted on a southwestern bypass of Cusseta, from south of the city to west of it.

The northern terminus of SR 1's proposed Summerville bypass was shifted to a point east-northeast of Trion.

[43][44] Five years later, in 1982, an eastern bypass of LaFayette, designated as SR 730, was proposed from southeast of the city to north-northeast of it.

A western bypass of Bremen, designated as SR 793, was proposed from south-southwest of the city to north-northwest of it.

[51][52] The next year, an eastern bypass of Blakely, designated as SR 838, was proposed from south-southeast of the city to north-northeast of it.

A southeastern bypass of Cuthbert, designated SR 847, was proposed from south-southwest of the city to north-northeast of it.

[59][60] In 1935, the Georgia General Assembly designated the portion of US 27/SR 1 from Columbus to its northern terminus as "Tennessee-Columbus Military Highway".

[61] In 1941, the Georgia General Assembly designated the portion of US 27/SR 1 from its southern terminus to Colquitt as the "De Soto Trail Highway".

[62] In 1952, the Georgia General Assembly designated the entirety of US 27/SR 1 as the "Martha Berry Highway", honoring a pioneer in education.

[67] In 2011, the Georgia General Assembly designated the entirety of US 27/SR 1 as the "Scenic Hometown Highway", for "tourism enhancement purposes".