Born into a family of an Uzbek laborer, he too began working at a ginnery in Kovunch at the age of fifteen.

He then became the Head of the Organizational Department of the Tashkent District Committee of the Communist Party of Uzbekistan and served in the position from 1928 to 1929.

From September 1931 to December 1934, Yusupov served as the Chairman of the Central Asian Bureau of the All-Union Trade Union Confederation in Tashkent.

From November 1936 to September 1937, Yusupov served as the People's Commissar of the Food Industry of the Uzbek SSR.

[5] Yusupov then served as the de facto head of state of the Uzbek SSR as the First Secretary of the Communist Party of Uzbekistan from 1937 to 1950.