A total of 52 clubs competed in this year's tournament, making it the most participants in the cup's history.
The first draw for the tournament took place on 24 September 2021, at Royal Spanish Football Federation (RFEF) headquarters in Las Rozas.
Alhama Cacereño Zaragoza Albacete Castellón Levante Las Planas Osasuna Córdoba Elche Juan Grande La Solana Pradejón Parquesol Granada The draw was held on 15 October 2021, in the RFEF headquarters in Las Rozas.
Santa Teresa Cacereño Zaragoza Alhama Córdoba Juan Grande La Solana Granada The draw was held on 4 January 2021, in the RFEF headquarters in Las Rozas.
[4] Málaga Dux Logroño Oviedo Espanyol Racing de Santander Alhama Juan Grande Granada Valencia Sporting de Huelva Athletic Club Betis Rayo Vallecano Eibar Alavés Villarreal The draw was held on 11 February 2022, in the RFEF headquarters in Las Rozas.