The winner is qualified for the Europa League play-off round.

92 teams participate in the first round, coming from all levels of competition.

Six additional teams will join in the second round, while the top six teams from the 2020–21 Danish Superliga enter in the third round.

[3] There are 92 teams: In the first round of the tournament, 92 teams took part, including 54 clubs from the various levels of the Denmark Series and below, all clubs from the Danish 3rd Division and Danish 2nd Division the 3rd-12th placed teams from the 2020-21 Danish 1st Division and the 11th-12th placed teams from the 2020-21 Danish Superliga.

Strandby-Elling-Nielstrup IF was the lowest ranked team, playing in the 10th tier.