In molecular biology, the ATP-grasp fold is a unique ATP-binding protein structural motif made of two α+β subdomains that "grasp" a molecule of ATP between them.

The reactions typically involve formation of acylphosphate intermediates.

These enzymes are involved in various metabolic pathways including purine biosynthesis, fatty acid synthesis, and gluconeogenesis.

[4] The ATP-grasp fold is evolutionarily conserved across different enzyme families and its presence is ubiquitous across prokaryotes and eukaryotes.

Some ATP-grasp enzymes are being studied as potential targets for antibiotics and anti-obesity drugs.