The story centers on the mission of a military squad sent to the zombie-infested Raccoon City to locate the whereabouts of Dr. Cameron, a female scientist conducting research on a new type of virus.

[2] In the Midwest of America, the citizens of Raccoon City have been transformed into zombies after becoming infected with the T-virus, a biological weapon secretly developed by the pharmaceutical company Umbrella.

A military squad—consisting of leader Claus (Masaki Aizawa) and his men Roger (Hiroto Torihata), Ed (Hideto Ebihara), Robert (Tadasuke Omizu) and Norman (Yoshiyuki Kaneko)—is sent into the city by the company.

Roger, who is a higher-up within Umbrella, reveals to the others that the true purpose of their mission was not to rescue Dr. Cameron, but to collect her research data regarding a new virus capable of regenerating genes.

The concept for Biohazard 4D-Executer was originally conceived by anime director Yasuhiro Imagawa, who proposed his idea of the film to Capcom, the game company responsible for the Resident Evil series.

[6] Former Capcom Production Studio 2 game designer and producer Keiji Inafune and Flagship co-founder Noboru Sugimura served as executive supervisors.

[7] The film marked director Koichi Ohata's first experience with computer-generated imagery (CGI), after his previous work on original video animations such as MD Geist, Cybernetics Guardian and Genocyber.

[11] For a more immersive presentation of the attraction, distributor Digital Amuse designed special cylindrical seats named "gimmick chairs", which can shake viewers and blow air on their necks.