It travels east from exit 16 along US 84/US 221/SR 38, then, in downtown Valdosta, makes a sharp left turn along US 41 Bus./SR 7 Bus.

It began at I-75 at what is today exit 37 (Old Quitman Road) and ran northeast to US 41, where it turned north.

In Sparks, it turned west along Colquitt Street where it ended at what is today exit 41.

The route begins at the Southwell Boulevard interchange and travels to the east-northeast for two blocks to an intersection with US 41/SR 7.

From there, they head on a curving route just west of Henry Tift Myers Airport south-southeast of Unionville, where SR 125 joins the concurrency.

Just past Sycamore Street, they leave Unionville and enter Tifton.

At the intersection with the eastern terminus of Forrest Avenue, they curve back to the north-northwest.

Just after an intersection with the western terminus of 22nd Street, they curve back to the north-northeast.

The four-highway concurrency then leaves the city limits of Cordele and winds around Crisp County–Cordele Airport.

North of there, it turns east onto Farmers Market Road, while US 41/SR 7/SR 90 head toward Vienna.

The route runs along Miami CR 25A and becomes Main Street further into the city.

It crosses US 36, then curves to the northeast before terminating at the northern section of exit 83.

I-75 Bus./US 224 makes a sharp turn at West Trenton Street and the business route ends at I-75 at exit 159, while US 224 continues westward toward Indiana.

The business loop in Pontiac runs through that city's downtown along a section of Woodward Avenue and a segment of roadway formerly used by M-24.

Each of the business loops connects to I-75 on both ends and runs through their respective cities' downtown areas.