In January 1986 Alder Valley North Limited, later renamed, The Berks Bucks Bus Company[4] took over the Bracknell, High Wycombe, Maidenhead, Newbury, Reading and Wokingham operations of Alder Valley as part of the preparation for privatisation of the National Bus Company, trading as "Beeline" once the name was changed in October 1986.

In 1993 Beeline purchased the Slough operations of Luton & District Transport, until 1986 the Slough depot of London Country Bus Services and later part of the London Country North West division.

[9] In December 2024 following the return to a national identity by First Group, First Beeline returned to trading as "First Berkshire and Thames Valley" [10] First Beeline currently operates bus services in and around Slough and to surrounding areas including Heathrow Airport, Maidenhead and High Wycombe.

[11] The company also operates the RailAir coach services connecting Heathrow Airport with surrounding major National Rail stations.

[13] The company also formerly operated the Green Line branded routes 702 and 703 between London, Heathrow Airport, Slough, Windsor, Legoland and Bracknell.