Formins

[12][13] The FH3 domain is less well conserved and is required for directing formins to the correct intracellular location, such as the mitotic spindle, or the projection tip during conjugation.

The GBD is a bifunctional autoinhibitory domain that interacts with and is regulated by activated Rho family members.

Mammalian Drf3 contains a CRIB-like motif within its GBD for binding to Cdc42, which is required for Cdc42 to activate and guide Drf3 towards the cell cortex where it remodels the actin skeleton.

The addition of the DAD to mammalian cells induces actin filament formation, stabilizes microtubules, and activates SRF mediated transcription.

This interaction is important in promoting the capture and stabilization of a subset of microtubules oriented towards the leading edge of migrating cells.