Gamalero (Gamaleri in Piedmontese) is a comune (municipality) in the Province of Alessandria in the Italian region Piedmont, located about 70 kilometres (43 mi) southeast of Turin and about 13 kilometres (8 mi) southwest of Alessandria.

Gamalero borders the following municipalities: Carentino, Cassine, Castellazzo Bormida, Castelspina, Frascaro, Mombaruzzo, and Sezzadio.

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