The route begins at the Alabama state line as a continuation of Alabama State Route 68.

The highway runs parallel to the Chattooga River, going through the towns of Chattoogaville and Lyerly before reaching Summerville.

In Summerville, SR 100 has a short concurrency with SR 114 until they both reach their northern terminus at an intersection with US 27/SR 1.

SR 114 is not part of the National Highway System, a system of roadways important to the nation's economy, defense, and mobility.

[2] The entire route is in Chattooga County.