It runs southeast-to-north through portions of Baker, Early, Calhoun, and Randolph counties.

In downtown Arlington, the three highways enter Calhoun County and cross a Norfolk Southern Railway track.

Two blocks later, SR 45 leaves the concurrency to the northeast on Morgan Road NE.

It continues heading north until it meets is northern terminus, an intersection with US 27/SR 1, just south of Cuthbert.

and the three truck routes begin at an intersection with SR 45/SR 62/SR 216 (Highland Avenue) in the central part of Arlington.