Insertion (genetics)

[citation needed] Based on CRISPR/Cas tools, different systems have already been developed to achieve specific functions.

[5] One limitation of current technology is that the size for DNA precise insertion is not large enough[6] to meet the demand for genome research.

Insertions can be particularly hazardous if they occur in an exon, the amino acid coding region of a gene.

Transcripts carrying the frameshift mutation may also be degraded through Nonsense-mediated decay during translation, thus not resulting in any protein product.

If translated, the truncated proteins frequently are unable to function properly or at all and can result in any number of genetic disorders depending on the gene in which the insertion occurs.