Kline Hollow Run begins in a valley in Jordan Township, Lycoming County, a short distance south of Richarts Grove.

It continues flowing south-southeast for more than a mile, receiving an unnamed tributary, before eventually gradually turning south.

Near the southern edge of Huckleberry Ridge, the stream reaches its confluence with Devil Hole Run.

[4] Kline Hollow Run is in the United States Geological Survey quadrangle of Benton.

[2] Additionally, the stream is located in the northern part of Pine Township and is approximately one mile (two kilometers) west of the community of Derrs.

[5] Kline Hollow Run is part of a Locally Significant Area in Columbia County.

[3] The Columbia County Natural Areas Inventory advises against extensive logging in the vicinity of Devil Hole Run.