Lesotho competed at the 2016 Summer Olympics in Rio de Janeiro, Brazil, from 5 to 21 August 2016.

A total of eight athletes, seven men and one woman, were selected to the Lesotho team to compete only in athletics, boxing, and mountain biking (the nation's Olympic sporting debut in Rio de Janeiro).

[2] Track sprinter Mosito Lehata led the Lesotho delegation as the lone returning Olympian from London 2012 and the nation's flag bearer in the opening ceremony.

Inkululeko Suntele had claimed his Olympic spot with a semifinal victory at the 2016 African Qualification Tournament in Yaoundé, Cameroon.

[5] Meanwhile, flyweight boxer Moroke Mokhotho had received a spare Olympic berth as the next highest-ranked boxer, not yet qualified, in the same meet, due to South Africa's decision not to accept spots through the continental qualifier.