It opens glutamate sensitive chloride channels in neurons and myocytes of invertebrates, leading to hyperpolarisation of these cells and blocking of signal transfer.

[3] Milbemycin oxime is active against a broad spectrum of nematodes.

[citation needed] The substance is often combined with other parasiticides to achieve a broader spectrum of action.

Such products include:[citation needed] The drug has been used in marine reef aquaria to control parasitic Tegastidae copepod infestations on captive hard coral colonies.

[5][6] The drug is usually tolerated well, but such side effects may occur such as vomiting, phlegming, and glassy eyes.