Magdalena Sibylle of Holstein-Gottorp (also spelled Magdalena Sibylla; 24 November 1631 at Gottorp Castle – 22 September 1719 in Güstrow) was a Duchess of Holstein-Gottorp by birth and by marriage Duchess of Mecklenburg-Güstrow.

Magdalene Sybille was the daughter of Duke Frederick III of Schleswig-Holstein-Gottorp (1597–1659) and his wife Marie Elisabeth of Saxony (1610–1684).

However, after the Mecklenburg-Güstrow line of Dukes had died out, the court in Güstrow lost its former glory and significance.

On 28 December 1654, she married Gustav Adolph, the ruling Duke of Mecklenburg-Güstrow.

This leads to a succession dispute that was settled by the Partition of Hamburg (1701), which redivided the interior of the Duchy of Mecklenburg among the surviving lines.