Matlacha ( /ˈmætləʃeɪ/ ⓘ MAT-lə-shay "MAT-la-shay"[4]) is a census-designated place (CDP) in Lee County, Florida, United States.

It is part of the Cape Coral-Fort Myers, Florida Metropolitan Statistical Area.

Matlacha is an "Old Florida" fishing village, home to many brightly colored art galleries, island boutiques, seafood restaurants, and traditional Floridian cottages.

County Road 78 (Southwest Pine Island Road) crosses the waterway at Matlacha, leading southwest 3 miles (5 km) to Pine Island Center and northeast through Cape Coral 12 miles (19 km) to North Fort Myers.

39.4% of all households were made up of individuals, and 19.3% had someone living alone who was 65 years of age or older.