Pine Island Center is an unincorporated community and census-designated place (CDP) located on Pine Island in Lee County, Florida, United States.

It is part of the Cape Coral-Fort Myers, Florida Metropolitan Statistical Area.

It is bordered to the north by Pineland and Bokeelia and to the south by St. James City.

CR-78 leads east across Little Pine Island to the mainland and 16 miles (26 km) to North Fort Myers, while Stringfellow Road runs the length of Pine Island, 7 miles (11 km) to Bokeelia at the north end and 8 miles (13 km) to St. James City at the south end.

23.7% of all households were made up of individuals, and 10.0% had someone living alone who was 65 years of age or older.