NeuN

There are in fact four protein products from the Fox-3 gene as a result of the presence or absence of two amino acid sequences coded by two exons.

[3] The inclusion or absence of 47 amino acids from exon 12 results in the ~46kDa and ~48kDa bands seen on SDS-PAGE gels, while the inclusion or absence of 14 amino acids from exon 15 produces two forms which are too similar in molecular size to be discerned on typical SDS-PAGE gels.

The Fox proteins are each about 46kDa in size, and each includes a central, highly conserved ~70 amino acid RRM or RNA recognition motif.

RRM domains are one of the most common in the human genome and are found in numerous proteins which bind RNA.

The three mammalian Fox proteins function in the regulation of mRNA splicing and bind specific RNA sequences.

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Rat hippocampus stained with antibody to NeuN/Fox-3 (green), myelin basic protein (red) and DNA (blue). Antibodies and image courtesy of EnCor Biotechnology Inc.
Antibody staining for NeuN/Fox-3 in the adult rat cerebellum in green. The NeuN/Fox-3 antibody binds to the small cerebellum granule cell neurons, which form a prominent layer. In contrast antibody to the close relative of NeuN/Fox-3, Fox-2, is shown in red. Fox-2 antibody stains Purkinje neurons and Golgi cell which are not recognized by the NeuN/Fox-3 antibody. The Purkinje cells form a layer above the much more abundant granule cells, and an occasional Golgi cell, also positive for Fox-2 but negative for NeuN/Fox-3, can be seen in the granule cell layer. DNA is shown in blue. Antibodies and image courtesy of EnCor Biotechnology Inc.