The western terminus of State Route 554 is at State Route 325 about 1 mile (1.6 km) north of Rio Grande, and just 1 block north of the State Route 325 interchange off of U.S. Route 35.

Its eastern terminus is at an intersection with State Route 7 in Cheshire, two blocks west of the Ohio River.

The entirety of State Route 554 is contained within Gallia County.

No changes of major significance have taken place to this state highway since it was first certified.

[3][4] The entire route is in Gallia County.