Phra Nakhon Si Ayutthaya Hospital (Thai: โรงพยาบาลพระนครศรีอยุธยา) is the main hospital of Phra Nakhon Si Ayutthaya Province, Thailand and is classified under the Ministry of Public Health as a regional hospital.

[1] After the death of King Chulalongkorn in 1910, the local authorities and citizens funded for a hospital in memory of King Chulalongkorn and this was known as "Panchamathirachuthit Hospital".

Most patients came by water transport, and thus in 1942, a canal was dug from the Chao Phraya River surrounding Ayutthaya's old town to the hospital.

In 1990, the hospital had a major renovation led by then Minister of Public Health, Boonpan Kaewattana under Chuan Leekpai's cabinet.

Aid such as water pumps were provided by King Bhumibol Adulyadej and King Vajiralongkorn (then crown prince) to reduce the effects of the flood.