This domain is also present in the molecules of phospholipase and several cytoplasmic tyrosine kinases such as Abl and Src.

Approximately 300 SH3 domains are found in proteins encoded in the human genome.

[7] The SH3 domain has a characteristic beta-barrel fold that consists of five or six β-strands arranged as two tightly packed anti-parallel β sheets.

Many SH3-binding epitopes of proteins have a consensus sequence that can be represented as a regular expression or Short linear motif: with 1 and 4 being aliphatic amino acids, 2 and 5 always and 3 sometimes being proline.

SH3 domain-mediated protein-protein interaction networks, i.e., SH3 interactomes, revealed that worm SH3 interactome resembles the analogous yeast network because it is significantly enriched for proteins with roles in endocytosis.