A total of 22 teams contest the league, 15 of which returned from the 2009–10 season, 4 of which have been promoted from Lega Pro Prima Divisione, and three relegated from Serie A.

On 25 April 2010, Livorno became the first team to be mathematically relegated to the league from Serie A;[1] on the same day, Novara put an end to a 33-year absence from the division by becoming Lega Pro Prima Divisione/A champions in advance of two weeks.

[3] On 9 May 2010, Atalanta were mathematically relegated into the Serie B;[4] on the same day, Portogruaro won the Lega Pro Prima Divisione/B title, thus ensuring themselves participation in the Italian second tier in a historical first team for the small Venetian club.

[8] On July 16, 2010 the Federal Council readmitted Ascoli and Portoguraro to Serie B championship, and excluded Ancona,[9] who subsequently announced their intention to appeal the decision to the Italian Olympic Committee.

On 28 October, the Venice prefecture signed the authorization that allowed Portosummaga to play its home matches at the Stadio Piergiovanni Mecchia, which had undergone renovation works during the summer, including the installation of a new stand.