Wolfhouse Run is a tributary of Little Fishing Creek in Columbia County, Pennsylvania, in the United States.

The stream then turns south and slightly west and begins to flow parallel to Wolfhouse Hollow Road for nearly a mile.

The stream then turns south-southwest, still flowing parallel to Wolfhouse Hollow Road, for a few tenths of a mile.

It then turns south-southeast for more than a mile and continues to flow parallel to Wolfhouse Hollow Road.

[1] Wolfhouse Run joins Little Fishing Creek 13.28 miles (21.37 km) upstream of its mouth.

[2] The watershed of the stream is in the Lower North Branch Susquehanna River drainage basin.

[5] Wolfhouse Run is in the United States Geological Survey quadrangle of Lairdsville.

[7] Wolfhouse Run was entered into the Geographic Names Information System on August 2, 1979.