The 1999–2000 English Premiership (known as the Allied Dunbar Premiership for sponsorship reasons) was the thirteenth full season of rugby union within the first tier of the English leagues, known as the Premiership.

The league was reduced from fourteen teams to twelve following London Scottish and Richmond both entering administration.

Points increased from 2 to 3 for a win after the World Cup in November 1999.

This was an attempt to ensure competitive balance in the leagues for teams while they were without their international players.

Note: Flags to the left of player names indicate national team as has been defined under World Rugby eligibility rules, or primary nationality for players who did not earn international senior caps.