The 1999–2000 UC Sampdoria season saw the club finish 5th in Serie B, a point away from promotion.

Sampdoria's kit was manufactured by Japanese company Asics and sponsored by Japanese gaming company Sega, who used their sponsorship to promote their console Dreamcast.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.

Players may hold more than one non-FIFA nationality.

Source: Worldfootball