The 2000–01 Slovak First Football League (known as the Mars superliga for sponsorship reasons) was the eighth season of first-tier football league in Slovakia, since its establishment in 1993.

AŠK Inter Slovnaft Bratislava were the defending champions.

A total of 10 teams was contested in the league, including 9 sides from the 1999–2000 season and one promoted from the 2.

HFC Humenné, FC Nitra, DAC 1904 Dunajská Streda, Dukla Banská Bystrica and Baník Prievidza) were relegated to the 2000–01 2.

These relegated teams were replaced by FK Matador Púchov.