The 2021 Prague Open (branded as the 2021 Livesport Prague Open for sponsorship reasons) was a professional women's tennis tournament played on outdoor hard courts at the TK Sparta Prague.

It took place in Prague, Czech Republic between 12 and 18 July 2021.

This was the first edition of the tournament held on outdoor hard courts, as opposed to clay courts in previous editions.

[1] This was Krejčíková's third WTA singles title of the year.

The following players received wildcards into the singles main draw: The following players received entry using protected rankings: The following player received entry as a special exempt: The following players received entry from the qualifying draw: The following players received entry as lucky losers: The following pairs received wildcards into the doubles main draw: