Union Omaha played their home games at Werner Park, located in Papillion, Nebraska, United States.

Union Omaha were the defending league champions, after defeating Greenville Triumph SC in the final the previous season.

[1] They also competed in the U.S. Open Cup for the first time, defeating two teams from Major League Soccer and reaching the quarterfinals, the farthest a team from USL League One has advanced in the tournament since the formation of the league.

In December 2021, Union Omaha announced an initial group of five players from last season's roster returning for the 2022 season.

[22] Source: USL League One[23] Numbers after plus–sign (+) denote appearances as a substitute.