DNAJC28

[11] The protein DNAJC28 is 388 amino acids long and contains a conserved N-terminal J (DnaJ) domain, which is critical for interaction with Hsp70s.

[12] Molecular weight and isoelectric point of human DNAJC28 without post-translational modification are 45.8 kDal and 9.57 pI, respectively.

J domains are highly conserved and are an integral part of protein translation, folding, translocation, and degradation through stimulating the ATPase activity of members of the Hsp70 family.

[18] There is a coiled-coil region from approximately amino acids 288 to 318 that is conserved throughout all listed orthologs (through fungi and plants).

[31] A mitochondrial targeting signal presequence traditionally has a high composition of arginine, a very low amount of negatively charged residues at the N-terminus, and forms an amphipathic helix with a positively charged side and a hydrophobic side opposite it.

[16] Heat Shock Protein genes are generally activated when the cell is exposed to stress, such as high temperature, infection, and low oxygen.

[39] The Hsp70/Hsp40 chaperone system works in proteostasis processes, which involves breaking down protein aggregations like a-synuclein which accumulates in Parkinson’s disease.

[40] A study found that damaging missense variants of DNAJC28 are likely related to sporadic late-onset Parkinson’s disease.

[41] DNAJC28 was found to be excessively expressed in the hippocampus of the lupus-prone mice model MRL/lpr during TWEAK (TNF-like weak inducer of apoptosis) activation, which is associated with the neuropsychiatric impacts of lupus.

[42] Overexpression of other genes in the DnaJ family has been shown to contribute to neuroprotective effects in multiple neurodegenerative disease models.

DNAJC28 human gene location with surrounding genes. [ 5 ] IFNGR2 encodes the beta chain of the gamma interferon receptor, and defects in it cause extreme immunodeficiency. [ 6 ] TMEM50B is hypothesized to be involved in endosome to vacuole transportation. Neighboring GART is involved in de novo purine synthesis. [ 7 ] SON encodes a protein that binds RNA, promotes pre-mRNA splicing, and recognizes a human Hepatitis B virus DNA sequence, repressing its core promoter activity. [ 8 ]
DNAJC28 transcriptional variants, numerically labeled on the left. The 2 exons are also labeled. Light green regions are untranslated while the dark green regions are the coding sequence.
Predicted DNAJC28 J domain annotated with helices and HPD motif. Helix locations and shape were predicted using E. coli DnaJ protein. HPD motif is highlighted.
DNAJC28 Evolutionary History comparing median Date of Divergence from Homo sapiens (millions of years) and Corrected Sequence Divergence for DNAJC28, Cytochrome C, Fibrinogen Alpha, and COG4. Corrected sequence divergence was calculated using the percent identity between the protein sequences of the different species to humans.
DNAJC28 iTasser Model 2. N-terminus is colored red. The predicted mitochondrial presequence is pictured in green (amino acids 7-39), light green is the NCBI listed DnaJ domain, yellow is Helix 1 (52-56), teal is Helix 2 (64-78), orange is the HPD motif, blue is Helix 3 (85-99), purple is Helix 4 (105-112).