Lillie's trichrome is a combination of dyes used in histology.
It was initially published by Ralph D. Lillie in 1940.
The resulting stains are black cell nuclei, brown cytoplasm, red muscle and myelinated fibers, blue collagen, and scarlet erythrocytes.
[3] Trichrome stains are normally used to differentiate between collagen and muscle tissues.
Some studies that benefit from its application include end stage liver disease (cirrhosis), myocardial infarction, muscular dystrophy, and tumor analysis.